ko.cwru.eduCase Transgenic And Targeting Facility

SOM CCCC CTSC Place An Order Transgenics Gene Targeting Chimeric Mice Rederivation CRISPR/Cas Sperm Cryopreservation In Vitro Fertilization Frozen Embryo Transfer New ES Cells Transfection Homozygosis Surgeries Success Rates PublicationsTimeline Cost Fundamentals CRISPR/Cas Fundamentals of Mouse Biology and Genetics Practical Mouse Colony Management Breeding Strategies (.pdf, Jax) Targeting Vector Design BAC Transgenic, Transgenic or Targeted Mice? Databases findmice.org informatics.jax.org eucomm.org creportal.org komp.org Cancer Mouse Repository Riken BioResource Center genome.ucsc.edu Ringwald/Eppig mouse database primer (.pdf) Protocols Breeding, Care And Maintenance Of Mice Plasmid Transgene DNA Purification Purification Of BAC DNA For BAC Transgenics Detecting Transgenes by PCR and Southern Blot Preparing Earpunch DNA For PCR Genotyping Preparing Mouse DNA For Southern Blots Performing Gavage on Mice Fostering Mice Gene Targeting Details Making ES Cells Details Links Breeding Mutants Genetics Commercial Services Pathology Groups Books Courses Cores Elsewhere We are leaders in CRISPR/Cas9 gene targeting . Our fees are low, and our success rates are high. New transgenic and targeted mice that we have created are featured in over 300 publications . We are supported by the Case Western Reserve University School of Medicine the Case Comprehensive Cancer Center , the Clinical and Translational Science Collaborative , the Research Institute for Children’s Health and by grants from the NIH, the Cystic Fibrosis Foundation and JobsOhio. We are a component of the FIND Pipeline , which translates gene-directed approaches into therapies for inherited disease. Name Role Office Phone Weihong Jiang Staff WRB 2514 368-2528 David LePage Staff WRB 2514 368-2528 Rachel Mann Manager WRB 2514 368-2528 Ron Conlon Director BRB 826 368-1826 Get up to $10,000 for pilot projects using our services from the Clinical and Translational Science Collaborative. Transgenics Targeting Chimeras Rederivation CRISPR/Cas Cryo IVF FET Other Transgenic and Targeted Mice Made by the Core 2024 Takita S, Jahan S, S Imanishi S, Harikrishnan H, LePage D, Mann RJ, Conlon RA, Miyagi M, Imanishi Y. Rhodopsin mislocalization drives ciliary dysregulation in a novel autosomal dominant retinitis pigmentosa knock-in mouse model. FASEB J. 2024 Apr 30;38(8):e23606. doi: 10.1096/fj.202302260RR. PMID: 38648465. Han X, Knauss EA, Fuente M, Li W, Conlon RA, LePage DF, Jiang W, Renna SA, McKenzie SE, Nieman MT. A Mouse Model of the Protease Activated Receptor 4 (PAR4) Pro310Leu Variant has Reduced Platelet Reactivity. J Thromb Haemost. 2024 Mar 18:S1538-7836(24)00161-2. PMID: 38508397. Vasudevan S, Senapati S, Pendergast M, Park PS. Aggregation of rhodopsin mutants in mouse models of autosomal dominant retinitis pigmentosa. Nat Commun. 2024 Feb 16;15(1):1451. PMID: 38365903; PMCID: PMC10873427. 2023 Wei T, Sun Y, Cheng Q, Chatterjee S, Traylor Z, Johnson LT, Coquelin ML, Wang J, Torres MJ, Lian X, Wang X, Xiao Y, Hodges CA, Siegwart DJ. Lung SORT LNPs enable precise homology-directed repair mediated CRISPR/Cas genome correction in cystic fibrosis models. Nat Commun. 2023 Nov 11;14(1):7322. PMID: 37951948 Esposito A, Klüppel M, Wilson BM, Meka SRK, Spagnoli A. CXCR4 mediates the effects of IGF-1R signaling in rodent bone homeostasis and fracture repair. Bone. 2023 166:116600 PMID: 36368465. Yang D, Wan X, Schwieterman N, Cavus O, Kacira E, Xu X, Laurita KR, Wold LE, Hund TJ, Mohler PJ, Deschênes I, Fu JD. MicroRNA-1 Deficiency Is a Primary Etiological Factor Disrupting Cardiac Contractility and Electrophysiological Homeostasis. Circ Arrhythm Electrophysiol. 2023 PMID: 38126205. 2022 Li Y, Liu Z, Zhao Y, Yang J, Xiao TS, Conlon RA, Wang Z. PD-L1 expression is regulated by ATP-binding of the ERBB3 pseudokinase domain. Genes Dis. 2022 Dec 8;10(4):1702-1713. PMID: 37397533 Kitt MM, Tabuchi N, Spencer WC, Robinson HL, Zhang XL, Eastman BA, Lobur KJ, Silver J, Mei L, Deneris ES. An adult-stage transcriptional program for survival of serotonergic connectivity. Cell Rep. 2022 Apr 19;39(3):110711 PMID: 35443166 Zhang XL, Spencer WC, Tabuchi N, Kitt MM, Deneris ES. Reorganization of postmitotic neuronal chromatin accessibility for maturation of serotonergic identity. Elife. 2022 Apr 26;11:e75970. PMID: 35471146 Massey W, Osborn LJ, Banerjee R, Horak A, Fung KK, Orabi D, Chan ER, Sangwan N, Wang Z, Brown JM. Flavin-Containing Monooxygenase 3 (FMO3) Is Critical for Dioxin-Induced Reorganization of the Gut Microbiome and Host Insulin Sensitivity. Metabolites. 2022 18;12(4):364. PMID: 35448550 Opoku E, Berisha S, Brubaker G, Robinet P, Smith JD. Oxidant resistant human apolipoprotein A-I functions similarly to the unmodified human isoform in delaying atherosclerosis progression and promoting atherosclerosis regression in hyperlipidemic mice. PLoS One. 2022 Feb 4;17(2):e0259751. PMID: 35120132 LaForce GR, Farr JS, Liu J, Akesson C, Gumus E, Pinkard O, Miranda HC, Johnson K, Sweet TJ, Ji P, Lin A, Coller J, Philippidou P, Wagner EJ, Schaffer AE. Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. Neuron. 2022 PMID: 35139363 Bohne P, Volkmann A, Schwarz MK, Mark MD. Deletion of the P/Q-type calcium channel from serotonergic neurons drives male aggression in mice. J Neurosci. 2022 Jul 18;42(34):6637–53. PMID: 35853721 2021 Gupta MK, Sahu A, Sun Y, Mohan ML, Kumar A, Zalavadia A, Wang X, Martelli EE, Stenson K, Witherow CP, Drazba J, Dasarathy S, Naga Prasad SV. Cardiac expression of microRNA-7 is associated with adverse cardiac remodeling. Sci Rep. 2021 11(1):22018. PMID: 34759299 Wei W, Liu Z, Zhang C, Khoriaty R, Zhu M, Zhang B. A common human missense mutation of vesicle coat protein SEC23B leads to growth restriction and chronic pancreatitis in mice. J Biol Chem. 2021 Dec 23:101536. PMID: 34954140 Vasu K, Ramachandiran I, Terenzi F, Khan D, China A, Khan K, Chechi A, Baleanu-Gogonea C, Gogonea V, Fox PL. The zinc-binding domain of mammalian prolyl-tRNA synthetase is indispensable for catalytic activity and organism viability. iScience. 2021 Feb 20;24(3):102215. PMID: 33748704 Haseeb A, Kc R, Angelozzi M, de Charleroy C, Rux D, Tower RJ, Yao L, Pellegrino da Silva R, Pacifici M, Qin L, Lefebvre V. SOX9 keeps growth plates and articular cartilage healthy by inhibiting chondrocyte dedifferentiation/osteoblastic redifferentiation. Proc Natl Acad Sci U S A. 2021 118(8) PMID: 33597301 Charrier A, Xu X, Guan BJ, Ngo J, Wynshaw-Boris A, Hatzoglou M, Buchner DA. Adipocyte-specific deletion of zinc finger protein 407 results in lipodystrophy and insulin resistance in mice. Mol Cell Endocrinol. 2021 PMID: 33285243 Wu J, Venkata Subbaiah KC, Jiang F, Hedaya O, Mohan A, Yang T, Welle K, Ghaemmaghami S, Tang WHW, Small E, Yan C, Yao P. MicroRNA-574 regulates FAM210A expression and influences pathological cardiac remodeling. EMBO Mol Med. 2021 13(2):e12710. PMID: 33369227 Xing G, Jing H, Yu Z, Chen P, Wang H, Xiong WC, Mei L. Membraneless condensates by Rapsn phase separation as a platform for neuromuscular junction formation. Neuron. 2021 PMID: 34033754 Han Y, Xia G, Srisai D, Meng F, He Y, Ran Y, He Y, Farias M, Hoang G, Tóth I, Dietrich MO, Chen MH, Xu Y, Wu Q. Deciphering an AgRP-serotoninergic neural circuit in distinct control of energy metabolism from feeding. Nat Commun. 2021 Jun 10;12(1):3525. PMID: 34112797 2020 Billi AC, Ludwig JE, Fritz Y, Rozic R, Swindell WR, Tsoi LC, Gruzska D, Abdollahi-Roodsaz S, Xing X, Diaconu D, Uppala R, Camhi MI, Klenotic PA, Sarkar MK, Husni ME, Scher JU, McDonald C, Kahlenberg JM, Midura RJ, Gudjonsson JE, Ward NL. KLK6 expression in skin induces PAR1-mediated psoriasiform dermatitis and inflammatory joint disease. J Clin Invest. 2020 130(6):3151-3157. PMID: 32155135 Tognatta R, Karl MT, Fyffe-Maricich SL, Popratiloff A, Garrison ED, Schenck JK, Abu-Rub M, Miller RH. 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